Introduction to Genome Mapping and Assembly

Assembling a complete genome from millions of fragmented DNA sequences is one of the most complex puzzles in modern biology. Without a structural blueprint, resolving repetitive regions is nearly impossible. This course provides a clear, step-by-step introduction to genome mapping, showing you how physical and genetic maps are used to guide high-quality genomic assembly. You will start with foundational terminology and the core principles of genomic architecture before exploring both classic and contemporary mapping methodologies. What you will learn: 1. Understand the critical differences between genetic, physical, and optical genome maps. 2. Explore traditional mapping techniques, including BAC clone libraries and radiation hybrid mapping. 3. Learn how to utilize closely related reference genomes to scaffold and guide new assemblies. 4. Discover modern scaffolding algorithms and long-read integration strategies. 5. Analyze and validate assembly quality using standard bioinformatics metrics. This text-based course is designed for beginners in bioinformatics, computational biology students, and software developers transitioning into the life sciences. No advanced background in genetics is required. Start reading today to build a solid foundation in genomic reconstruction.